At the moment, Indonesia still relies heavily on fossil fuels to cover its domestic energy demands. However, one of the main causes of the rise in greenhouse gas emissions is the nation's reliance on fossil fuels for energy damage the environment. An alternate energy source called bioethanol. The fermentation process of natural ingredients with the help of microorganisms, Bioethanol produced from biomassa in this study is empty oil palm bunches because of the cellulose content of 45.59% which can be converted into second-generation bioethanol. Poses for making bioethanol empty oil palm bunches through the process of pretreatment, hydrolysis, fermentation and distillation. The purpose of this study is to determine the characteristics of bioethanol produced by empty oil palm bunch waste. Bioethanol characteristic testing refers to test standards determined by SNI 7390:2008/2013, This study produced ethanol content of 4.92% in 6-day fermentation did not meet the specified standards, methanol content produced 0.4% in 10-day fermentation and had met the specified standards, water content in 4-10 days fermentation produced 25.56%-30.72% did not meet the specified standards, denaturant content in 10-day fermentation produced 9% had met the specified standards. Furthermore, the acidity in fermnation 4-10 days produces 0.6 mg / l - 0.1 mg / l has met the specified standards, chloride ion levels in fermentation 4-10 days produce 0.8 mg / l - 0.2 mg / l has met the specified standards, sulfur content in fermentation 4-10 days produces 0.5 mg / l - 0.2 mg / l has met the specified standards, the sap content in 10 days fermentation produces 0.4 mg / l has met the specified standards.